Sugarcane Bud Chip Encapsulation for ex vitro Synthetic Seed Formation

Role in Bud Formation

RHO? encodes a Rho-type small GTPase of the yeast Saccharomyces cereuisiae. We isolated temperaturesensitive alleles and a dominant active allele of RH03. Tsrho? cells lost cell polarity during bud formation and grew more isotropically than wild-type cells at nonpermissive temperatures. In contrast, cells carrying a dominant active mutant RHO? displayed cold sensitivity, and the cells became el...

In Vitro Flower Bud Formation, Plant Regeneration and Morphogenetic Studies in Local Scented Cultivar of Rosa indica

In vitro propagation of rose (Rosa indica) as well as rose breeding is a valuable economic enterprise and has received highest importance globally including Indian subcontinent. The development of new cultivars for commerce is faced with new challenges. In vitro micropropagation protocols of varieties of roses have been established but very few reports on the clonal propagation of local cultiva...

In vitro shoot proliferation and in vitro and ex vitro root formation of Pyrus elaeagrifolia Pallas

Shoot-tip cultures of Pyrus elaeagrifolia Pallas, an important gene source for drought and chlorosis resistance in pear rootstock breeding, were established from a wild mature tree originated from seed. Murashige and Skoog basal medium supplemented with different concentrations of benzyladenine (BA) singly or in combination with auxin was used in the study. In the initial culture, the highest p...

GTP/GDP exchange by Sec12p enables COPII vesicle bud formation on synthetic liposomes.

The generation of COPII vesicles from synthetic liposome membranes requires the minimum coat components Sar1p, Sec23/24p, Sec13/31p, and a nonhydrolyzable GTP analog such as GMP-PNP. However, in the presence of GTP and the full complement of coat subunits, nucleotide hydrolysis by Sar1p renders the coat insufficiently stable to sustain vesicle budding. In order to recapitulate a more authentic,...

Bud Formation and Shoot Development In Vitro: Observations on Stem and Bud Explants of Psychotria punctata (Rubiaceae)